Then, 400?l lysing buffer 1 concentrate (BD pharmingen, catalog#555899) buffer was added into 80?l blood samples for 5?min at room temperature to remove red blood cells. significant decrease after the first cycle of anti-PD-1 treatment. Besides, M-MDSCs levels in the PR group were maintained at a low level in the following therapeutic cycles. Consistently, Tregs … Continue reading Then, 400?l lysing buffer 1 concentrate (BD pharmingen, catalog#555899) buffer was added into 80?l blood samples for 5?min at room temperature to remove red blood cells